The spiritual environment has become an important part of the present scenario and a diverse and dynamic field that continues to evolve as people seek to find deeper meaning, connection, and purpose in their lives. The present study attempted to study the effect of intergenerational conflict among adolescents, young adults, and older adults living in a spiritual environment. The population included samples (N=90) among which 30 are adolescents, 30 are young adults and 30 are older adults. The samples were collected from ashrams in Haridwar and Ahmedabad. The data were collected through Intergenerational Conflict Scale developed by Dr. Roma Pal (1987). Using a one-way ANOVA test, the study aimed to determine if there was a significant difference in the effect of intergenerational conflict on spirituality across the different age groups. The null hypothesis was not accepted, indicating that there was no significant difference in the effect of intergenerational conflict among the three age groups living in spiritual environments. While intergenerational conflict is a common phenomenon in spiritual environments, this study suggests that its effect on spiritual well-being may be similar across age groups. The results finding suggested that the impact of intergenerational conflict on spirituality in spiritual environments is consistent across different age groups, highlighting the need for effective communication and understanding between generations to reduce conflict and promote spiritual well-being. Promoting communication between generations to foster a positive and inclusive spiritual environment.