This study aimed to examine the relationship between level of self-esteem and aggressive behavior among Isra university students. Further step, to find out whether gender, Job of guardian, and the student's general academic grade statistically effect in self-esteem and aggressive behavior. Sample of the study consisting of (200) participants (100 male & 100 female) were randomly selected from Isra university- Jordan. For purpose of data collection, the researchers adopted the self-esteem scale by [1], and aggressive behavior scale by [2]. The finding of the study revealed statistically negative relationship between level of self-esteem and aggressive behavior of university students. Moreover, there were no statistically significant difference in level of self-esteem and aggressive behavior of Isra university students according to gender, Job of guardian, and the student's general academic grade of participants variables.